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Plasmid DNA Purification

17013 - Fast DNA-spin™ Plasmid DNA Purification Kit, 200 columns

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$235.00

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Fast DNA-Spin Plasmid DNA Purification Kit is designed for rapid separation and purification of plasmid DNA from E. Coli using silica membrane spin-type columns with an alkali lysis buffering system. High quality plasmid DNA can be obtained in only 10 minutes. Ice incubation and centrifugation are not necessary. With the alkali lysis method, the pH change can be visually observed in real time due to the included Lysis Viewer, a lysis and neutralization indicator. The purified plasmid DNA can be used directly for a variety of downstream applications.

Features

  • Designed for purification of DNA from 1-4ml overnight cultures of E. Coli
  • Protocol takes only 10 minutes
  • Plasmid DNA can be extracted with relatively low amounts of buffer
  • Ice incubation and centrifugation are not necessary

Applications

  • Restriction enzyme digestion
  • Sequencing
  • Library screening
  • Ligation and transformation
  • In vitro translation
  • Transfection of robust cells

Contents

  • M1 buffer (resuspension buffer), 60ml
  • M2 buffer (lysis buffer), 60ml
  • M3 buffer (neutralization buffer), 80ml
  • Washing buffer, 50ml
  • Elution buffer, 30ml
  • Lysis Viewer, 300 µl
  • RNase A (10 mg/ml), 600 µl
  • Spin column x 200
  • Collection tube x 200

Literature