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RealMOD™ Green W² 2x qPCR mix

25350 - RealMOD™ Green W² 2x qPCR mix, 100 reactions

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RealMOD Green W2 2x qPCR mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SYBR Green I dye. It contains all the components necessary to perform qPCR: Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and SYBR Green I dye. The user simply needs to add water, template and primers. Hot start
DNA Polymerase is activated by a 5 minutes incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during qPCR setup. The kit has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.

Features

  • Contains all components necessary to perform qPCR
  • High specificity and reproducibility
  • High efficiency for targets with low copy number
  • Excellent sensitivity and almost no signal-to-noise due to primer dimers
  • Excellent qPCR results in templates with high GC contents 

Applications

  • Real-time PCR: Detection and quantification of DNA and cDNA targets
  • Gene expression profiling: Gene knockdown verification
  • Microbial detection: Viral load determination
  • Array validation: SNP genotyping

Contents

  • RealMOD™ Green W2 2x qPCR mix*, 1ml
*Contains Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and SYBR Green I dye


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