RealMOD Green W2 2x qPCR mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SYBR Green I dye.
This solution contains all the components necessary to perform qPCR: Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and SYBR Green I dye. The user simply needs to add water, template and primers.
HotStart DNA Polymerase is activated by a 5 minute incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during qPCR setup. The kit has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.
Features
- Contains all components necessary to perform qPCR
- High specificity and reproducibility
- High efficiency for targets with low copy number
- Excellent sensitivity and almost no signal-to-noise due to primer dimers
- Excellent qPCR results in templates with high GC contents
Applications
- Real-time PCR: Detection and quantification of DNA and cDNA targets
- Gene expression profiling: Gene knockdown verification
- Microbial detection: Viral load determination
- Array validation: SNP genotyping
Contents
- RealMOD Green W2 2x qPCR mix*, 1ml
*Contains Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and SYBR Green I dye
Literature