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Enzymes

GE-NUC10700 - HS-Nuclease, recombinant Endonuclease

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HS-Nuclease is a recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Bezonase®) produced in E. coli using a proprietary process.

This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1 - 4 bases in length. HS-Nuclease is a highly purified homodimer of 27 kDa subunits that has exceptionally high specificity and is free of protease activity.

HS-Nuclease is ideal for digesting nucleic acids and reducing viscosity during protein purification and sample preparation.

 

Features

  • Encoded by the same gene as Bezonase®
  • >99% purity
  • Free of protease activity
  • High specificity
  • Does not interfere with any downstream processes not involving nucleic acids

 

Applications

  • Reducing viscosity of cell lysates 
  • Removing nucleic acid contaminations from sample preparations

 

Sizes

  • GE-NUC10700-01 - HS-Nuclease, recombinant Endonuclease, 50,000 U
  • GE-NUC10700-02 - HS-Nuclease, recombinant Endonuclease, 2 x 50,000 U

 

Literature