MobiSpin G-Columns pre-packed with Sephadex® G-50 resin remove small molecules from DNA/RNA, such as salt ions, and are therefore most suitable for buffer exchange.
The Sephadex G-50 matrix is a hydrophilic matrix that swells in buffer and acquires its chromatographic properties under wet conditions. In this state, beads have a pore size of 700 Da and allow hydrated salt ions or dNTPs (dye-labeled or radiolabeled) to enter the pores while DNA/RNA under 20 bases and most other biomolecules stay outside. .
The MobiSpin G-Columns are closed with a screw cap and the tip is a fixed snap-off plug.
Features
- Pre-packed with Sephadex® G-50 resin and equilibrated in STE buffer (10mM Tris/HCl pH 8.0, 100 mM NaCl, 1 mM EDTA)
- Sample purification can be completed in less than 4 minutes
- Creates reproducible results with simplified protocols
- Numerous samples can be processed simultaneously
- No sample dilution
- Compatible with laboratory standards
Applications
- Nucleic acid purification
- Desalting and Buffer exchange
- Removal of dye terminators or unincorporated labeled nucleotides
Sizes
- SCO500 - MobiSpin column prefilled with G-50 x 20
- SCO510 - MobiSpin column prefilled with G-50 x 100
Literature