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Microscopy - Slides & Dishes

801X-16 - Imaging Chamber, TC-Surface, 16/box

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These Imaging Chambers are designed for high resolution live cell microscopy techniques. With microscope slides as the bottom structure, the chambers can be used with long distance objectives. They are suitable for in-situ hybridization technologies as well as for a wide range of fixation and staining protocols.
 
The chamber bodies and lids are made of a high performance, acetone-resistant polymer that can withstand temperatures up to 90°C. Cell adhesion, spreading and distribution on top of the glass bottom is improved due to proprietary plasma surface modifications. The chambers are sealed against the glass bottom by a biological inert silicone adhesive. At the end of the experiment, the glass can be detached from the polymer chambers with minimal glue residue. 
 

Specification

Cat# 8011-16

Cat# 8012-16

Number of wells per chamber

1

2

Chamber height

10 mm

10 mm

Imaging area per well

10.84 cm2

5.04 cm2

Total volume per well

11 ml

5.5 ml

Suggested working volume per well

2000 µl

1000 µl

Total chamber length

58 mm

58 mm

Total chamber width

26 mm

26 mm

 

Features

  • Extraordinary planarity of the imaging plane, even during temperature shifts
  • High temperature stability, suitable for FISH
  • High chemical resistance, fixation with acetone enabled
  • Bottom glass can be detached with minimal glue residues
  • Imaging Chambers are delivered with lid and are placed on a slide tray plate
  • Microscope slides have TC surface
 

Applications

  • Laser scanning confocal microscopy (LSM)
  • Total internal reflection fluorescence (TIRF)
  • Differential interference contrast (DIC)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence resonance energy transfer (FRET)
  • Fluorescence recovery after photobleaching (FRAP)
  • Low intensity fluorescence
  • Fluorescence in-situ hybridization (FISH)
  • Immunohistology
 

Formats

  • 8011-16 - Imaging chamber, 1 well per chamber, 16/box
  • 8012-16 - Imaging chamber, 2 wells per chamber, 16/box
 

Literature