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Ni-IDA columns for His-Tag Purification

PR-HTK000 - Ni-IDA columns for His-Tag Purification

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Ni-IDA columns with 1g silica-based resin provide a fast and convenient tool for purification of recombinant polyhistidine-tagged proteins by gravity flow. The form-stable silica matrix is pre-charged with Ni2+ ions and allows purification on the principle of Immobilized Metal Ion Chromatography (IMAC). Binding of proteins is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. The chelating group of the Ni-IDA resin is based on IDA (iminodiacetic acid), which enables strong and efficient binding of target protein onto the IMAC matrix. 
 
In contrast to traditional IDA matrices, MoBiTec's Ni-IDA is an optimized matrix with low density of IDA ligands. This non-saturating surface concentration of IDA eliminates almost all non-specific interactions of contaminating host proteins with the absorbent. As a result, MoBiTec's Ni-IDA provides higher target protein purity.
 

Features

  • Used for easy, fast, and cost-effective routine purification 
  • Columns are pre-packed and ready-to-use
  • High binding capacity - maximum is 90mg protein per column
  • Protein recovery rate is greater than 80%
  • Improved target specificity by optimized silica-based Ni2+ - IDA matrix
  • Imidazol-free loading and washing buffer
  • Columns can be stored long term when kept dry
 

Applications

  • Purification of recombinant polyhistidine-tagged proteins by gravity flow
 

Sizes

  • PR-HTK004 - Ni-IDA columns x 4
  • PR-HTK010 - Ni-IDA columns x 10
 

Literature