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Bacillus subtilis Protein Expression System

BSUB PGRAC100 VECTORS - Bacillus subtilis Pgrac100 vectors, 10 µg

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B. subtilis vector



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The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.
 
The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.
 
The B. subtilis Pgrac100 expression vectors are improved derivatives of the Pgrac01 expression vectors. These vectors are B. subtilis E. coli shuttle vectors were constructed for cloning in E. coli and high level expression of heterologous proteins with B. subtilis.
 
All Pgrac100 vectors use the strong promoter preceding the groESL operon of Bacillus subtilis with improved regulatory elements fused to the lac operator, allowing their induction by IPTG. Nucleotides were optimized at the conserved regions of the groESL promoter including the UP element, the −35 and the −15 region. The combination of these changes into one promoter increased the amount of recombinant proteins accumulating intracellularly by roughly 30% of the total cellular protein of B. subtilis. In addition, the target proteins can also be efficiently expressed in E. coli in some cases.
 
Multiple vectors are offered:
  • pHT253 (PBS013) - for intracellular protein production, with N-terminal encoded 8xHis tag
  • pHT254 (PBS014) - for intracellular protein production, with C-terminal encoded 8xHis tag
  • pHT255 (PBS015) - for intracellular protein production, with C-terminal encoded Strep tag
  • pHT1469 (PBS018) - for secretory protein production, with improved signal sequence of amyQ for protein export
 
Please note this product requires a license for non-academic institutions. Please contact us for licensing information.
 

Features

  • Strong promoter with improved regulatory elements
  • Enhanced amount of expressed recombinant proteins
  • Inducible protein expression
  • Non-pathogenic
  • No significant bias in codon usage
  • Useful for the construction of metagenomic libraries
 

Applications

  • Intracellular production of recombinant proteins in B. subtilis
 

Vectors

  • PBS013 - pHT253 vector, 10 µg
  • PBS014 - pHT254 vector, 10 µg
  • PBS015 - pHT255 vector, 10 µg
  • PBS018 - pHT1469 vector, 10 µg
 

Literature

Publications