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E. coli Expression Systems

P123T - PCR cloning Vector p3T

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The p3T vector provides a flexible system for the direct cloning of PCR products. It allows the cloning of PCR products via multiple dT overhangs. Utilizing the restriction site PflMI, three dT overhangs are produced. After polyadenylating the PCR fragment with Terminal Deoxynucleotidyl Transferase, the PCR fragment can be cloned with high efficiency via a multiple dA extension. By using p3T, less amplified DNA is required. A SmaI site is present to reduce vector background and MscI sites flank the insert for optimal excision. Blue/white selection by alpha-complementation can be used with the p3T vector.
 

Features

  • Clones PCR products via multiple dA extensions
  • Results in higher cloning efficiencies than with single dA/dT overhangs
  • Requires less amplified DNA
 

Applications

  • Cloning PCR products
 

Contents

  • p3T, PCR Product Cloning Vector, lyophilized DNA, 5 µg
 

Literature