The vector pG-20-hH2A.1 is a ready-to-use vector for expression of EGFP-histone hH2A.1 fusion protein. These fusion proteins can be used in modern fluorescence microscopy techniques (FRET, FRAP, FCS, RICS, FCCS, and F3H) to determine localization, binding, dynamics, protein-protein interactions and protein-protein proximities. The cipher in the vector name indicates the linker length of the fusion proteins.