Description
i-Taq DNA Polymerase is a thermostable DNA polymerase of 94 kDa. It is expressed in E. coli by cloning the polymerase gene of Thermus aquaticus (strain YT1). It removes the E. coli-derived protein and DNA which can act as a contaminant in PCR. DNA can be amplified up to 5 Kb. The included buffer has been optimized to show the best polymerase activity regardless of template type or reaction conditions.
- 94 KDa thermostable DNA polymerase
- High purity Taq DNA Polymerase
- Removal of E. coli-derived proteins and DNA that may act as PCR sources
- Optimized buffer
- Genomic DNA PCR
- RT-PCR
- Direct sequencing related PCR
- T/A vector cloning
- LOH or MSI analysis related PCR
- i-Taq DNA Polymerase (5U/ml), 500 units
- 10x PCR Buffer (with 20mM MgCl2), 1ml
- 10x MgCl2-free PCR Buffer, 1ml
- 10mM dNTPs (2.5mM/each), 1ml
- 25mM MgCl2, 1ml