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{{/_source.additionalInfo}}Reverase (M-MuLV, RNase H minus)
10,000 units (Cat# 105100)
Description
Reverase is a modified enzyme purified from a plasmid in E. coli strain that directs the synthesis of Moloney Murine Leukemia virus (M-MuLV) reverse transcriptase. M-MuLV reverse transcriptase is an RNA or DNA directed DNA polymerase.
Reverase can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single stranded DNA as a template. This enzyme has been genetically altered to remove associated RNase H activity. Removal of the RNase H activity resulted in an increase of full-length cDNA products. Subsequent amplification of cDNA can be completed with Taq DNA Polymerase.
- Directs the synthesis of M-MuLV reverse transcriptase
- Genetically modified to remove RNase H activity, resulting in an increase of full-length cDNA products
- Molecular weight is 69 kDa
- Reverse Transcription
- Detection of viruses in combination with PCR amplification of cDNA
- 105100 – Reverase, 10,000 units
- 105250 – Reverase, 50,000 units
Also Included:
- Incomplete RT Reaction Buffer (5x)
- Complete RT Reaction Buffer (5x)
- MgCl2 100 mM
- DTT 100 mM
