Polymer-based transfection is an optimized delivery system that allows high efficiency with low cellular stress due to improved delivery mechanisms. Polyfection has been revolutionized with the design and synthesis of a novel patented Cationic Hydroxylated Amphiphilic Multi-block Polymer (CHAMP). This bi-functional copolymer is biocompatible, ionizable and pH sensitive. Formed by three moieties, it combines and introduces three synergistic notions:
- The concept of “passing through the membranes barriers” due to its charge, pH-sensitive and hydrophobic properties.
- The idea of “stealth transfection” where DNA is protected, masked and supported all the way to its nuclear uptake.
- The concept of biocompatibility due to biodegradable and cleavable moieties
is a powerful transfection reagent based on CHAMP technology. With Helix-IN, cellular stress is reduced, viability preserved and efficiency enhanced.