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{{/_source.additionalInfo}}ReadyTector® Pure
ReadyTector® Pure, Includes ReadyTector® Solution (without secondary antibody) and 10x Wash Buffer, 2 x 40ml (Cat# 701 040)
Description
One-Step-Detection-Solution for Immunoblots enabling immunodetection in a single step!
ReadyTector® Pure features a speedy, efficient, and effective protocol! ReadyTector® Pure allows you to perform blocking, primary antibody binding, and secondary antibody binding simultaneously, greatly simplifying and accelerating the workflow Despite this fast single-step incubation process, ReadyTector® minimizes background and enables clear, publication-quality signal detection.
ReadyTector® Pure is compatible with multiple detection labels, including horseradish peroxidase (HRP), alkaline phosphatase (AP), fluorescent dyes, and nanoparticles as well as multiplexing approaches. Optimal primary and secondary antibody concentrations must be empirically determined.
ReadyTector® Pure is designed to suppress low and medium affinity binding events, which are a common source of background. Consequently, a slight signal reduction may occur if polyclonal antibodies (which generally also contain low- and medium-affinity binding components) are used. In this case, the amount of high-affinity antibodies can be raised by moderately increasing the antibody concentration to achieve the desired signal strength again. The unwanted low and medium-affinity binding will remain suppressed.
Additional bottles of 10x wash buffer can be purchased separately.
- Easy one-step protocol enabling immunodetection in one step!
- Fast procedure with quick, but effective results
- Reduces background for clear, sharp publication quality bands
- Versatile solution
- Western blotting
| Cat# | Format | Size |
| 701 040 | ReadyTector® Pure, Includes ReadyTector® Solution (without secondary antibody) and 10x Wash Buffer, 2 x 40ml | 40ml x 2 |
| 701 120 | ReadyTector® Pure, Includes ReadyTector® Solution (without secondary antibody) and 10x Wash Buffer, 2 x 120ml | 120ml x 2 |
| 701 500 | ReadyTector® Pure, Includes ReadyTector® Solution (without secondary antibody) and 10x Wash Buffer, 2 x 500ml | 500ml x 2 |
All formats include a bottle of Readytector® solution and a bottle of 10x wash buffer.
Related Categories
Immunoassay OptimizationBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
