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HYPE-5 High Yield Protein Expression

HY00000 - HYPE-5 Transfection Kit


Starting at: $239.00

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HYPE-5 + Hype Blast

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HYPE-5™ Transfection Kit is designed to achieve High Yield Protein Expression in mammalian cells.
This kit has been developed for maximum efficiency in HEK293 and CHO cells growing in suspension (flask, spinner and bioreactor) and cultivated in chemically defined medium. It is suitable for other cells as well. These efficient transient expression systems provide a fast, economical alternative to the stable expression systems, which can be costly and time-consuming. Scaling up to larger volumes for production is straightforward with simple and cost-efficient handling steps.
The HYPE-5 kit is composed of HYPE-5 transfection reagent (a Lipofection reagent) and HYPE-Blast reagent, included for improving protein production in CHO cells.
Figure 1: HYPE-5™ transfection kit achieves higher protein yield than popular commercial transfection reagents in CHO (A) and HEK (B) cells in suspension. Secreted Alkaline Phosphatase (SEAP) was produced by transient transfection using HYPE-5 (HEK293 cells) or HYPE-5 + HYPE-Blast (CHO cells), LP or FHD transfection reagents according to the manufacturer's instructions. Day 3, 5 and 7 supernatants were collected and SEAP activity was measured.


  • Provides high yield recombinant protein production
  • Suitable for both HEK293 and CHO cells growing in suspension
  • Compatible with any synthetic or regular media used for protein production
  • Completely animal origin free
  • Ideal for bioreactors, spinners and flasks


  • Bioproduction and biopharmaceutical manufacturing
  • Protein production


HY01500 - Suitable for 0.5-1 L of cell culture
  • HYPE-5 reagent, 1.5ml
  • HYPE-Blast, 5ml
HY03000 - Suitable for 1-2 L of cell culture
  • HYPE-5 reagent, 1.5ml x 2
  • HYPE-Blast, 5ml x 2
HY15000 - Suitable for 5-10 L of cell culture
  • HYPE-5 reagent, 15ml 
  • HYPE-Blast, 50ml
HY30000 - Suitable for 10-20 L of cell culture
  • HYPE-5 reagent, 15ml x 2
  • HYPE-Blast, 50ml x 2