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{{/_source.additionalInfo}}DreamFect Transfection Reagent
500µl (Cat# DF40500)
Description
DreamFect™ Transfection Reagent uses TEE (Triggered Endosomal Escape) technology to achieve efficient nucleic acid delivery into cells. It contains both a lipidic portion and a cationic portion, allowing the association of nucleic acids with the reagent and permitting nucleic acid delivery into cells.
Due to its unique properties, DreamFect™ releases large amounts of DNA into the cytosol, increasing DNA nuclear delivery. Consequently, DreamFect™ is highly efficient at low doses of nucleic acids. Fewer cationic lipids are used and the potential secondary effects are avoided.
Figure 1: DreamFect transfection efficiency in various cell lines. 1x105 cells were transfected with 0.5 μg / well of pEGFP plasmid DNA in 24-well plates. Percentage of transfected cells was measured 24h post transfection by flow cytometry.
Figure 2: Comparison of DreamFect efficiency with other transfection reagents. Cell lines (1x105 cells / well) were transfected with 1 μg of pLuc plasmid DNA and 3 μl of DreamFect Gold reagent per well in a 24-well plate. The other transfection reagents were assayed according to the manufacturer’s instructions.
- High efficiency and reproducibility
- Can be used with all nucleic acids and all cells including suspension cell lines
- Ideal for all nucleic acids applications
- Serum-compatible
- Non-toxic and biodegradable
- Simple, rapid protocol
- Transfection of cell lines
- DF40500 – DreamFect Transfection Reagent, 500 µl (125-250 transfections)
- DF41000 – DreamFect Transfection Reagent, 1ml (250-500 transfections)
- DF45000 – DreamFect Transfection Reagent, 5ml (1250-2500 transfections)
DreamFect Gold Transfection Reagent
DreamFect Stem Transfection Reagent
EcoTransfect Transfection Reagent