Lullaby siRNA Transfection Reagent

Name: Lullaby siRNA Transfection Reagent
SKU: LL70500
Price: 250 USD
Availability: InStock

500µl (Cat# LL70500)

Sales Price$250.00

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Description

Lullaby is the ideal siRNA transfection reagent for gene silencing. This reagent uses TEE Technology (Triggered Endosomal Escape), a mechanism that combines and exploits the properties of cationic lipids and polymers to achieve extremely efficient DNA delivery into cells. Lullaby has been successfully tested on numerous cell lines, reaching up to 90% gene silencing with high reproducibility and very low toxicity.

RNA interference is a powerful technique to shut down genes' expression in cells and organisms. Short RNA duplexes are extremely selective by interacting and inducing the degradation of their specific mRNA targets, and thereby inhibit the resulting protein production. Lullaby® siRNA Transfection Reagent introduces the siRNA duplexes in a variety of cells with a very high efficiency, leading to exceptional knockdown effects with low doses of siRNA.

Figure 1: GFP silencing in HeLa cells. GFP-expressing HeLa cells (A) seeded in a 24-well plate were transfected with 1µl Lullaby + 5nM (33.75ng) siRNA (B) . GFP extinction was monitored 72h post-transfection by fluorescence microscopy.

Figure 2: GFP silencing in various cell lines with Lullaby GFP-expressing cells were seeded on a 24-well plate and transfected with 10nM (67.5ng) siRNA associated with 2µl of Lullaby. GFP extinction was monitored 72h post-transfection by flow cytometry.

+ Features

Exceptional siRNA delivery efficiency and gene silencing (>90%)
Suitable for all siRNA applications (co-transfection, endogenous gene silencing)
Effective gene silencing at multiple siRNA concentrations including low doses (
risk of off-target effects
Simple, rapid protocol
Powerful across a broad spectrum of cells and confluency (from 20 to 90%)
Serum compatible and non-toxic
Adapted to high-throughput siRNA screening