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Magnetofection Magnetic Transfection

GL00000 - Glial-Mag Transfection Reagent

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Starting at: $288.00

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Glial-Mag is designed to reach optimal transfection efficiency with microglial cell lines and primary cells.
 
The Glial-Mag kit includes the Glial-Mag reagent, a specific magnetic nanoparticles formulation, and Glial-Boost, a booster designed to enhance transfection efficiency. A starter kit is also available that includes an 8x12cm Magnetic Plate
Figure 1: 0.2µg of pVectOZ-GFP (BV2/Primary) and 0.4µg of pVectOZ-GFP (N9/N13) were complexed with Glial-Mag at a 3.5:1 ratio and transfection was performed according to the standard protocol. After 24h, GFP+ cells were analyzed by Flow cytometry.
 

Figure 2: Transfection of BV2. Complexes of pVectOZ-GFP encoding DNA and Glial-Mag were prepared as described in the standard protocol. Other commercial transfection reagents were used as recommended by the manufacturers. After 24h, GFP+ cells were analysed by fluorescence microscopy.

Figure 3: Transfection of BV2. 0.2 µg pVectOZ-GFP were complexed with Glial-Mag at a 3.5:1 ratio and transfection was performed in presence or not of Glial-Boost. After 24h, GFP+ cells were visualized under fluorescent microscopy and % of GFP+ cells was determined by Flow cytometry.

Features

  • Developed specifically for DNA transfection into microglial cell lines and primary cells
  • Low amount of nucleic acid - minimized toxicity
  • High level of nucleic acid compaction
  • Easy and straightforward protocol
  • Compatible with any culture medium
 

Applications

  • Transfection of microglial cells line BV2, N9, N13, HMO6, MG-5, SIM-A9, primary microglia
  • Transient and stable transfection
 

Formats

  • GL00250 - Glial-Mag Transfection Reagent, 250 µl + Glial-Boost, 3ml
  • GL00500 - Glial-Mag Transfection Reagent, 500 µl + Glial-Boost, 6ml
  • KGL0250 - Glial-Mag Transfection Reagent, 250 µl + Glial-Boost, 3ml + Magnetic Plate