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{{/_source.additionalInfo}}SilenceMag siRNA Delivery Reagent
200µl (Cat# SM10200)
Description
SilenceMag siRNA Delivery Reagent is designed for siRNA transfection using Magnetofection technology. This reagent gives reliable high protein knockdown at very low doses of siRNA in a variety of types of cells (primary cells, hard-to-transfect cells and cell lines). SilenceMag is serum-compatible and non-toxic.
SilenceMag must be used with a magnetic plate. Two starter kits are available that include a Super Magnetic Plate (8x12cm) or a 96-Place Magnetic Plate (8x12cm). Magnetic plates can also be purchased separately.
Figure 1: GFP silencing in HeLa cells. GFP-expressing HeLa cells seeded in a 96-well plate were transfected with 0.5 µL of SilenceMag and 4 or 10nM siRNA (corresponding to 10.8 or 27 ng respectively). GFP extinction was monitored 72h post-transfection by fluorescence microscopy.
Figure 2: LacZ silencing in various cell lines with SilenceMag. Various cells were co-transfected in 96-well plates with 100ng of pLacZ plasmid complexed to 0.1µL of PolyMag and either 1 or 5nM of siRNA associated with SilenceMag. b-galactosidase expression was monitored after 48h.
- High protein knockdown efficiency
- Used for all siRNA applications
- No off-target effects
- Serum-compatible and non-toxic
- Simple, rapid protocol
- siRNA transfection of primary and hard-to-transfect cells
- Gene silencing of siRNA, dsRNA, shRNA
- Sequential and simultaneous transfections
- Endogenous gene silencing
- SM10200 - SilenceMag siRNA Delivery Reagent, 200 µl (400 assays)
- SM10500 - SilenceMag siRNA Delivery Reagent, 500 µl (1,000 assays)
- SM11000 - SilenceMag siRNA Delivery Reagent, 1ml (2,000 assays)
- SM13000 - SilenceMag siRNA Delivery Reagent, 3ml (6,000 assays)
- KC30300 - Starter Kit: 200ul SilenceMag + Magnetic Plate
- KC30396 - Starter Kit: 200ul SilenceMag + 96-Place Magnetic Plate
PolyMag Nucleic Acid Delivery Reagent
FluoMag Red Fluorescent Magnetofection Reagent, 100µl
Starter Kit with Universal Magnet: CombiMag + PolyMag + PolyMag Neo + SilenceMag
In Vivo SilenceMag Transfection Reagent