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Transfection for CRISPR

RMC70500 - RmesFect™ CRISPR Transfection Reagent, 500 µl

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RmesFect™ CRISPR Transfection Reagent is specifically designed for mRNA/gRNA transfection with high efficiency and low toxicity. This reagent can be used in a large variety of cells. 
 
mRNA transfection has several advantages over plasmid DNA delivery. With mRNA transfection, there is no need for nuclear uptake, as mRNA translation into proteins occurs in the cytoplasm. mRNA transfection provides faster protein expression than DNA transfection with no genomic integration. Additionally, protein expression occurs in a totally promoter-independent manner.
 
 

Figure 1: DC24 cells were transfected with mRNA encoding for GFP and RFP (0.25µg each) using RmesFect CRISPR according to the standard protocol. Images were analyzed under microscopic fluorescence 24H after transfection.

Features

  • Achieves RNA delivery in a variety of cells
  • Ready-to-use; no need for additional buffer
  • Low amount of nucleic acid minimizes toxicity
  • Protects RNA against degradation
  • Compatible with any culture medium
 

Applications

  • Genome Editing with CRISPR/Cas9
 

Contents

  • RmesFect™ CRISPR Transfection Reagent, 500 µl