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Gilthead Seabream Antibodies

153529 - Anti-IgT [Z55F8*C3]


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Cat# 153529
Applications ELISA IHC WB
Antigen/Gene or Protein Targets IgT, Immunoglobulin Heavy Constant Tau
Synonyms IgT
Reactivity Gilthead seabream, Meagre and Lumpsucker
Relevance Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. This Anti-IgT antibody provides a useful tool to monitor vaccine performance in fish and will assist in the development of future vaccines, especially mucosal vaccines. Target species: Meagre (Argyrosomus regius), gilt-head (sea) bream (Sparus aurata), Lumpsucker (Cyclopteridae), Large yellow croaker (Larimichthys crocea).
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide
Positive Control ELISA - fish sera, peptide immunogen; Western Blot, Sera; IHC, Formalin-fixed, paraffin-embedded head kidney, gill and spleen tissues
Subclass IgG
Myeloma Used P3X63Ag8.653
Notes Recommended Usage Conditions: ELISA- neat; Western blot- neat; IHC- 1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0
Research Area Aquaculture


Image: Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Positive membranous immunostaining is observed in Meagre gill tissue using Anti-IgT [Z55F8*C3]. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).


For Research Use Only.