Cat# | 151622 |
Applications | ELISA, IHC, WB |
Antigen/Gene or Protein Targets | Activated MMP9 (matrix metalloproteinase 9) |
Reactivity | Human and Rat |
Relevance | Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumor invasion and metastasis. |
Host | Mouse |
Immunogen | Ovalbumin-conjugated synthetic peptide; acetylated-FQTFEGDLK |
Positive Control |
IHC: Formaldehyde-fixed, wax-embedded oesophegal adenocarcinoma Western Blot: Activated recombinant MMP-9, 400ng per lane |
Subclass | IgG1 kappa |
Myeloma Used | P3X63Ag8.653 |
Recommended Growing Conditions | Dulbecco’s media containing L-Glutamine and 20% Fetal Bovine serum (DH20) |
Strain | Balb/c |
Notes | Western Blot: not reactive with proenzyme of MMP-9, or proenzyme or activated forms of MMP-2 |
Research Area | Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Protein Degradation |
References | Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model. Europe PMC ID: 26136898 Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form. Europe PMC ID: 9851689 |
Image: Immunohistochemical localisation of activated MMP-9 in oesophageal cancer using mAb 4A3.
For Research Use Only