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{{/_source.additionalInfo}}Anti-MMP9 [2C3]
(Cat#: 151621)
Description
| Cat# | 151621 |
| Applications | IHC, IF, IP, WB |
| Antigen/Gene or Protein Targets | Human matrix metalloproteinase 9 (MMP-9) |
| Reactivity | Human |
| Relevance |
Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumor invasion and metastasis. Expression of MMP9 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide; KLGLGADVAQVT |
| Positive Control |
IHC: formalin-fixed, paraffin-embedded lung containing intra-alveolar macrophage. Western Blot: rhMMP-9, 400 ng per lane |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing L-Glutamine and 20% Fetal Bovine serum (DH20) |
| Strain | Balb/c |
| Notes |
IHC: a recommended dilution of 1/32 and pretreatment of antigen retrieval: microwave for 10 minutes at 950W in 0.01M sodium citrate buffer, pH 6.0. Western Blot: not reactive with MMP-1, -2, -3. A recommended dilution of 1/5. |
| Research Area | Adhesion, Cancer, Cardiovascular, Epigenetics & Nuclear Signaling |
| References |
Molecular evidence for the chemosensitizing efficacy of liposomal curcumin in paclitaxel chemotherapy in mouse models of cervical cancer. Europe PMC ID: 21317920 The matrix metalloproteinase/tissue inhibitor of matrix metalloproteinase profile in colorectal polyp cancers. Europe PMC ID: 19635101 Profiling markers of prognosis in colorectal cancer. Europe PMC ID: 16489072 Matrix metalloproteinase/tissue inhibitors of matrix metalloproteinase phenotype identifies poor prognosis colorectal cancers. Europe PMC ID: 15623598 Matrix metalloproteinases and their inhibitors in gastric cancer. Europe PMC ID: 9824606 Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form. Europe PMC ID: 9851689 Matrix metalloproteinase-1 is associated with poor prognosis in oesophageal cancer. Europe PMC ID: 9771478 |
Image: Wax embedded IHC using 2C3 at a dilution of 1/32.
For Research Use Only.
