Cat# | 151852 |
Applications | ELISA, IF, WB |
Antigen/Gene or Protein Targets | Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41 |
Reactivity | Virus |
Relevance |
Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive. The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B. |
Host | Mouse |
Immunogen | Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100°C for 2 mins. |
Positive Control |
Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2. First antibodies: Line1 RSV convalescent human sera. Lane 2: 4-18 antibody. Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells |
Subclass | IgG2a |
Myeloma Used | P3X63Ag8.653 |
Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine). |
Strain | Balb/c |
Research Area | Virology |
References | Immunoblot analysis of the human antibody response to respiratory syncytial virus infection. Europe PMC ID: 3572364 |