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Pathogen Antibodies

151852 - Anti-RSV Nucleoprotein VP41 [4-18]


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Cat# 151852
Applications ELISA, IF, WB
Antigen/Gene or Protein Targets Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41
Reactivity Virus
Relevance Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.

There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA.

Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.
Host Mouse
Immunogen Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100°C for 2 mins.
Positive Control Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Line1 RSV convalescent human sera. Lane 2: 4-18 antibody. Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells
Subclass IgG2a
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
Strain Balb/c
Research Area Virology
References Immunoblot analysis of the human antibody response to respiratory syncytial virus infection. Europe PMC ID: 3572364


For Research Use Only.