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Pathogen Antibodies

151851 - Anti-RSV Subgroup B Phosphoprotein [4-14]

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Cat# 151851
Applications ELISA IF WB
Antigen/Gene or Protein Targets Human Respiratory Syncytial (RS) virus phosphoprotein VP32
Reactivity Virus
Relevance Immunoblot assays performed in parallel using as antigen 19 clinical RS virus isolates of subgroups A and B (isolated between 1972 and 1984) showed that this antibody reacted only with the phosphoprotein of RS virus subgroup B (Gimenez et al, 1986).

RS virus subgroups A and B circulate concurrently in the human population. Infections with RS virus subgroup A are thought to produce more severe disease than infections with RS virus subgroup B. Consequently, it is beneficial for patient management to know early during an RS virus infection whether the patient is infected with RS virus subgroup A or B. Thus, this antibody is relevant for as a diagnostic tool to determine, soon after infection, the specific RS virus subgroup responsible for the RS virus infection.
Host Mouse
Immunogen The immunogen was gradient-purified RSN-2 virus (subgroup B) that was then treated with 0.1% SDS at 100°C for 2 min. The procedure used to produce this antibody is described in Gimenez et al. (1984).
Positive Control Immunoblot: Partial purified PVM (lane 2). Partial purified BRS virus (lane 3). Gradient-purified RSN-2 virus (lane1 & 4): 5ug/lane. First antibody: 4-14 antibody (lanes 1,2,3 & 4). Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells
Subclass IgM
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
Strain Balb/c
Notes Diagnostic kit proposal:

Antibody : 3-5-18 4-14
Cat number : 151850, 151851

Control samples:
RS A2 Subgrp A + -
RSN-2 Subgrp B + +

Patient # 1 sample + -
Patient # 2 sample + +

Results:

Patient # 1 was infected with RSV Subgrp A Patient # 2 was infected with RSV Subgrp B.
Research Area Virology
References

Immunoblot analysis of the human antibody response to respiratory syncytial virus infection. Europe PMC ID: 3572364

Antigenic variation between human respiratory syncytial virus isolates. Europe PMC ID: 3517224

Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. Europe PMC ID: 6202832

 

Image: The antigens used were gradient-purified RSN-2 virus in lanes 1 and 4, partial purified PVM (lane 2) and BRS virus (lane 3). First antibodies: Lane 1: RS virus convalescent human sera; Lanes 2, 3 and 4: antibody 4-14. The identity and molecular weight of the protein target of this antibody was validated by including within the immunoblot assay (as a marker) a convalescent serum sample from a RS virus infected patient. The protein specificities of the antibodies present in the convalescent serum are described in Gimenez et al. (1987).

 

For Research Use Only.