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{{/_source.additionalInfo}}Bacillus subtilis Pgrac01 Expression Vector
pHT01 (PBS001)
Sales Price$1,106.00
Please note this product requires a license for commercial companies. Please contact us for details.
Description
The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.
The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.
The B. subtilis Pgrac01 expression system provides an easy-to-handle tool for high yield intracellular protein production with B. subtilis and for secretion of proteins into the culture medium in large amounts. Expression of the gene of interest is regulated by the Pgrac01 promoter fused to the lac operator, allowing the induction by addition of IPTG.
Vectors
- pHT01 (PBS001) – IPTG inducible expression vector for intracellular protein production.
- pHT43 (PBS002) – secretion vector; derivative of pHT01 with signal sequence of amyQ for protein export.
- pHT08 (PBS003) – derivative of pHT01 with C-terminal encoded 8xHis tag.
- pHT09 (PBS004) – derivative of pHT01 with C-terminal encoded Strep tag.
- pHT10 (PBS005) - derivative of pHT01 with C-terminal encoded c-Myc tag.
- pHT1464 (PBS010) – secretion vector; derivative of pHT01 with improved signal sequence of amyQ for extended protein export.
Bacillus subtilis control vectors are also available.
Please note these products require a license for commercial companies. Please contact us for licensing information.
- Developed for convenient cloning in E. coli and high level expression of heterologous proteins with B. subtilis
- Structurally and segregationally stable
- Non-pathogenic
- No significant bias in codon usage
- Can secrete functional extracellular proteins directly into the culture medium
- Useful for the construction of metagenomic libraries
- Intracellular or secretory protein production with B. subtilis
| Cat# | Vector | Features |
| PBS001 | pHT01 | All subsequent vectors are derivatives of pHT01 |
| PBS002 | pHT43 (secretion) | amyQ signal sequence for protein export |
| PBS003 | pHT08 | C-terminal 8xHis tag |
| PBS004 | pHT09 | C-terminal Strep tag |
| PBS005 | pHT10 | C-terminal c-Myc tag |
| PBS010 | pHT1464 (secretion) | Improved amyQ signal sequence for extended protein export |
All vectors are supplied as lyophilized DNA (10µg).
Bacillus subtilis Host Strain, 1ml
Bacillus subtilis Control Vector