Smart One RT-PCR Master Mix

Cat#: 105610
105610-smart-one-rt-pcr-mastermix
Sales Price$211.00
Size
100 Reactions
Description

Smart One RT-PCR Master Mix is a 2x ready-to-use Master Mix for all probe-based RT-qPCR applications

This one-tube, one-step master mix efficiently performs Reverse Transcription of RNA into cDNA and subsequent PCR amplification. With high sensitivity, a low concentration of template RNA is required. The convenient master mix format minimizes pipetting steps and contamination risks, as only the primer and RNA are required. Blue dye is included in the liquid mix for visual pipetting.

Smart One RT-PCR Master Mix contains:

  • Reverse Transcriptase
  • HotStart Taq polymerase
  • dNTPs
  • MgCl2
  • Reaction buffer
  • RNAsin
  • Stabilizer 

The Reverse Transcription step is directed by a Reverse Transcriptase with a heat stability up to 55°C. PCR amplification of synthesised cDNA is performed by a HotStart Taq DNA polymerase.

 

  • Convenient, ready-to-use format
  • High sensitivity; low concentration of sample RNA template is required
  • Minimizes contamination risks and pipetting steps
  • Contains blue dye for convenient visual pipetting
  • Reverse Transcriptase is stable up to 55°C
  • RT-qPCR
  • Analysis of RNA samples with Real-Time PCR for probe-based PCR assays
  • Smart One RT-PCR Master Mix (2x), 1ml, 100 reactions

Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.

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