PolyMag Neo Magnetofection Reagent is an optimized formulation of PolyMag for a higher gene expression level in primary, hard-to-transfect and cell lines. It is specifically designed for high transfection efficiency. It is composed of magnetic nanoparticles coated with cationic molecules. This reagent must be used with a magnetic plate.
Figure 1: PolyMag Neo transfection efficiency in various cell lines. 1x105 cells were transfected with 0.5 μg / well of pEGFP plasmid DNA in 24-well plates. Transfections were performed with 0.5 μl / well of polyMag Neo reagent. Percentage of transfected cells was measured 24h post transfection by flow cytometry.
Figure 2: PolyMag Neo transfection efficiency in various cell lines. 1x105 cells were transfected with 0.5 μg / well of pEGFP plasmid and 0.5 μl of PolyMag Neo reagent in 24-well plates. EGFP expression was monitored 24h after transfection by fluorescence microscopy.