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{{/_source.additionalInfo}}Anti-NSE [NSEP1]
(Cat#: 151866)
Description
| Cat# | 151866 |
| Applications | ELISA, IHC, WB |
| Antigen/Gene or Protein Targets | Gamma-isozyme of human enolase (NSE) |
| Reactivity | Human |
| Relevance | Neuron specific enolase (NSE, or gamma-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P1: aa’s 416-433 - LGDEARFAGHNFRNPSVL |
| Positive Control | IHC: formalin-fixed, paraffin-embedded nerve tissue sections; western blot: isozyme of human enolase; 50-100 ng per lane |
| Subclass | IgG1 kappa |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin. |
| Strain | Balb/c |
| Notes | Not reactive with -isozyme of enolase |
| Research Area | Cancer, Cell Type or Organelle Marker, Developmental Biology, Metabolism, Neurobiology, Stem Cell Biology |
| References |
Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies. Europe PMC ID: 8254105 A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific gamma isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences. Europe PMC ID: 1629611 |
For Research Use Only
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