
Cat# | 153312 |
Applications | ELISA, FACS, IHC, WB |
Antigen/Gene or Protein Targets | Immunoglobulin D Heavy Constant Delta |
Synonyms | IgD, Immunoglobulin D Heavy Constant Delta |
Reactivity | Salmon, Rainbow Trout and Brown Trout |
Relevance | Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. Moreover, our Anti-IgD antibody provides a useful tool to monitor vaccine performance in fish and will assist in the: development of future vaccines. |
Host | Mouse |
Immunogen | Ovalbumin-conjugated synthetic peptide. Peptide immunogen is 80% identical to IgD channel catfish (Ictalurus punctatus), 70% identical to Japanese halibut (Paralichthys olivaceus), 70% identical to Chinese perch (Siniperca chuatsi). Antibody potentially could cross-react with turbot (Scophthalmus maximus) 66% similarity to peptide immunogen. |
Positive Control |
ELISA: immunized fish sera FACS: Peripheral blood lymphocyte, head kidney cells |
Subclass | IgG1 |
Myeloma Used | P3X63Ag8.653 |
Strain | Balb/c |
Notes | Recommended Usage Conditions: ELISA - neat; Western blot - neat; IHC - neat, antigen retrieval microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0; FACS - 1/2 dilution |
Research Area | Aquaculture |
Image: Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Positive membranous immunostaining is observed in infected trout kidney using Anti-IgD [Z56P1H5*G6].
For Research Use Only.
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