3D-Fect 3D Transfection Reagent

3D-Fect™ is a 3D Transfection Reagent specifically developed to directly transfect cells cultured in 3D scaffolds. It is suitable for all types of scaffolds and cells.

3D matrices add a third dimension to the cells' environment and can induce significant differences in cellular phenotype and behavior. Scaffold-based 3D matrices activated with 3D-Fect™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. These transfections can be studied in tissue engineering and regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth and other areas.

 

Figure 1: 3D-Fect allows transfection of a variety of cells (cell lines and primary cells) into 3D-scaffolds: Collagen-based and collagen-derived scaffolds were pre-loaded with complexes formed by 3D-Fect™ (ratio 4:1) and 1 µg of either pVectOZ-GFP (green pictures) or RFP plasmids (red pictures). Cells were then seeded and observed under fluorescence microscopy after 48 h.

 

Figure 2: Alkaline phosphatase secretion of cells transfected into 3D Scaffold: Collagen-derived scaffolds were pre-loaded with complexes formed by 1 µg of pVectOZ-SEAP and 4 µl of 3D-Fect reagent. Cells were then seeded and secreted alkaline phosphatase (SEAP) concentration was measured after 48 h.