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{{/_source.additionalInfo}}RmesFect mRNA Transfection Reagent
500µl (Cat# RM20500)
Description
RmesFect mRNA Transfection Reagent is specifically designed for in vitro mRNA transfection with high efficiency and low toxicity.
mRNA transfection is advantageous over plasmid DNA delivery in that it does not require nuclear uptake and it is not integrative. It also provides faster protein expression than DNA expression. RmesFect is based on TEE technology (Triggered Endosomal Escape) that combines and exploits the properties of cationic lipids and polymers. The cationic design of RmesFect protects mRNA from degradation for efficient transport directly into the cytosol.
Figure 1: Several cell lines were transfected with 0.5 µg of mRNA encoding GFP protein and RmesFect transfection reagent (ratio 2:1 for RAW264.7,3:1 for COS7 and CHO-K1 and 4:1 for HeLa cells). After 20 min of incubation at room temperature, complexes were added onto the cells in a dropwise manner. 24 H after, transfection efficiency was measured by FACS analysis.
Figure 2: Several cell lines were transfected with mRNA encoding GFP protein and RmesFect transfection reagent. 24 H after, transfection efficiency was measured by fluorescence microscopy.
- Specifically designed for mRNA transfection
- High efficiency and low toxicity
- Can be used with all types of cells
- Protects mRNA against degradation
- Compatible with serum and any culture medium
- Transfection of mRNA in primary cells and cell lines
- mRNA vaccine development, regenerative medicine, cell reprogramming, iPs generation, etc.
- RM20500 - RmesFect Transfection Reagent, 500 µl (125-250 transfections)
- RM21000 - RmesFect Transfection Reagent, 1ml (250-500 transfections)
- RM25000 - RmesFect Transfection Reagent, 5ml (1250-2500 transfections)
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DreamFect Gold Transfection ReagentBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.