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{{/_source.additionalInfo}}i-pfu DNA Polymerase (High Fidelity)
Cat#: 25181
Description
i-pfu DNA Polymerase is a typical heat-resistant proof-reading enzyme with 3'- 5' exonuclease activity. It has high fidelity and guarantees an error rate of 1x10-6 (one error rate per 13,000 base pairs). The proofreading activity of i-pfu DNA Polymerase results in PCR products with more accurate base sequences than when using Taq DNA Polymerase. When using i-pfu DNA Polymerase, DNA fragments longer than Taq DNA Polymerase (up to 30 kb) can be amplified. Due to these properties, i-pfu DNA Polymerase is widely used in experiments in which high accuracy is required, such as gene cloning and expression. Unlike Taq DNA Polymerase, this i-pfu DNA Polymerase produces blunt-end amplification products.
- High accuracy makes it possible to amplify long DNA templates
- PCR products have more accurate bases
- Low error rate of 1x10-6
- Used in blunt-end cloning
- Ideal for experiments requiring high accuracy
- PCR
- Blunt Cloning
- Template amplification for DNA sequence analysis
- i-pfu DNA Polymerase (2.5 U/ml), 250 units
- 10x PCR Buffer (with 15 mM Mg2+), 1ml
- 10x Mg2+-free PCR Buffer, 1ml
- 10mM dNTPs (2.5 mM/each), 500μl
- 25mM Mg2+, 1ml
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Maxime PCR PreMix Kit, pfuBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
