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{{/_source.additionalInfo}}DreamFect Stem Transfection Reagent
500µl (Cat# ST30500)
Description
DreamFect™ Stem is an original, simple and efficient reagent specially developed to transfect multipotent and embryonic stem cells with high transfection efficiency and superior transgene expression level.
DreamFect™ Stem transfection reagent is based on TEE Technology (Triggered Endosomal Escape) that combines and exploits the properties of cationic lipids and polymers to achieve an extremely efficient DNA delivery into stem cells. It is a powerful reagent with improved cytoplasmic release and better biodegradability. DreamFect™ Stem allows DNA delivery in a variety of stem cells.
DreamFect™ Stem exhibits high transfection efficiency on human adult multipotent stem cells such as MSC (Mesenchymal Stem Cells), AdSC (Adipose derived Stem Cells), AFSC (Amniotic Fluid Stem Cells), and KG1a (leukemic stem-like cells) as well as embryonic stem cells.
Figure 1: Several human stem cells (MSC, AdSC, AFSC, KG1a) were transfected with 0.5 μg of pVectOZ-GFP plasmid DNA and 1.5 μl per well of DreamFect™ Stem reagent per well in a 24-well plate. Percentage of GFP positive cells were measured 48h post transfection by flow cytometry.
Figure 2: Adherent stem cells (MSC, AdSC, AFSC) were transfected with 0.5 μg of pVectOZ-GFP plasmid DNA and 1,5 μl of Dreamfect™ Stem per well in a 24-well plate. Transfection efficiency was assessed by fluorescence microscopy 48h post transfection (Magnification x 200).
- High transfection efficiency for multipotent Stem cells
- Minimized toxicity due to reagent biodegradability and low DNA amount required
- Cell phenotype and differentiation potential are not affected
- Serum Compatible
- Simple, rapid protocol
- Transfection of stem cells with high transgene expression level
- ST30500 – DreamFect™ Stem, 500 µl (125-250 transfections)
- ST31000 – DreamFect™ Stem, 1ml (250-500 transfections)
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RmesFect STEM mRNA Transfection ReagentBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.