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{{/_source.additionalInfo}}Anti-CDJ1 | chloroplast DnaJ homolog 1
(Cat#: AS06 179)
Description
- Immunogen: Fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 100 µl
- Reconstitution: For reconstitution add 100 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: 1: 3000 (WB)
- Expected | apparent MW: 40 | 40 kDa
- Confirmed reactivity: Chlamydomonas reinhardtii
- Not reactive in: Higher plants
- CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.
- Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.
- Western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl
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