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{{/_source.additionalInfo}}Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5
(Cat#: AS07 212)
Description
- Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC proteins including Arabidopsis thaliana VDAC1 UniProt: Q9SRH5 , TAIR: AT3G01280, VDAC2 UniProt F4K3R8-1 , TAIR: AT5G67500, VDAC3 UniProt: Q9SMX3-1, TAIR: AT5G15090, VDAC4 UniProt: Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt: Q9M2W6-1, TAIR: AT3G49920
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: 2D Blue Native PAGE (2D BN-PAGE), Western blot (WB)
- Recommended dilutions: 1 : 500 (IL), 1 : 5000, 2-30 µg protein/lane (WB)
- Expected | apparent MW: 29 kDa (for Arabidopsis thaliana)
- Confirmed reactivity: Arabidopsis thaliana, Amaranthus palmeri, Beta vulgaris, Brassica oleracea var. botrytis, Brassica rapa subsp. rapa, Citrus sinensis, Fortunella margarita Swingle, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrium patens, Zea mays
- Not reactive in: Chlamydomonas reinhardtii, Glycine max, diatoms, Saccharomyces cerevisiae
- VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.
- Soria et al. (2024).Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants. Current Plant Biology Volume 37, March 2024, 100322.Wittmann at al. (2024). Dual plastid targeting of Protoporphyrinogen Oxidase 2 in Amaranthaceae promotes herbicide tolerance. Plant Physiol. 2024 Feb 8:kiae062.doi: 10.1093/plphys/kiae062.Bao et al. (2023). Aberrant accumulation of ceramides in mitochondria trigger cell death requiring autophagy in Arabidopsis. J Exp Bot . 2023 Dec 9:erad456.doi: 10.1093/jxb/erad456. Xue et al.(2023). The PtdIns3P phosphatase MtMP promotes symbiotic nitrogen fixation via mitophagy in Medicago truncatula. iScience. 2023 Sep 15;26(10):107752.doi: 10.1016/j.isci.2023.107752. Belykh et al. (2022) Responses of genes of DNA repair, alternative oxidase, and pro-/antioxidant state in Arabidopsis thaliana with altered expression of AOX1a to gamma irradiation. Int J Radiat Biol. 2022;98(1):60-68. doi: 10.1080/09553002.2022.1998712. Epub 2021 Nov 11. PMID: 34714725.Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.Tarasenko et al. (2020). Plant mitochondrial subfractions have different ability to import DNA. Theor. Exp. Plant Physiol. doi.org/10.1007/s40626-020-00167-wGarmash et al. (2020). Altered levels of AOX1a expression result in changes in metabolic pathways in Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation. Plant Sci. 2020 Feb;291:110332. doi: 10.1016/j.plantsci.2019.110332.Bai et al. (2019). Overexpression of soybean GmPLD? enhances seed oil content and modulates fatty acid composition in transgenic Arabidopsis. Plant Science Volume 290, January 2020, 110298.Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.
- Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
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