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{{/_source.additionalInfo}}Anti-Deg1 | chloroplastic DegP-type serine protease 1
(Cat#: AS07 246)
Description
- Immunogen: Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Total IgG. Protein G purified in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 360 µg
- Reconstitution: For reconstitution add 200 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 500/5 ug thylakoid fraction (WB)
- Expected | apparent MW: 46.6 | 36 kDa
- Confirmed reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.
- Zienkiewicz et al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.
- Unspecific reaction at ca. 30 kDa and ca. 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease, The band at the right MW does contain Deg1 by our MS analysis
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