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{{/_source.additionalInfo}}Anti-LOX-C | Lipoxygenase (chloroplastic)
(Cat#: AS07 258)
Description
- Immunogen: recombinant Arabidopsis thaliana protein, loop (aa 257-450) UniProt: P38418, TAIR: At3g45140
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 100 µl
- Reconstitution: For reconstitution add 100 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 50 000 (WB)
- Expected | apparent MW: 102 | 97 kDa
- Confirmed reactivity: Arabidopsis thaliana, Vitis vinifera
- Not reactive in: Chlamydomonas reinhardtii
- Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Alternative name of the antigen: Lipoxygenase 2
- Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941Cecchini et al. (2018). Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. Mol Plant Microbe Interact. 2018 Aug 29. doi: 10.1094/MPMI-07-18-0185-R. (antibody used on LOX2 mutant plant)Pilati et al. (2015). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14:87. doi: 10.1186/1471-2229-14-87.
- Aweak band at around 84 kDa is detected as a probable result of cross-reaction with another lipoxygenase
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