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{{/_source.additionalInfo}}Anti-L7/L12 | Ribosomal protein
(Cat#: AS08 331)
Description
- Immunogen: Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 200 µl
- Reconstitution: For reconstitution add 200 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 1: 10 (IF), 1 : 1000 (WB)
- Expected | apparent MW: 11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)
- Confirmed reactivity: Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
- Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
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