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{{/_source.additionalInfo}}Anti-CBP80 | nuclear cap-binding protein subunit 1
(Cat#: AS09 531)
Description
- Immunogen: Recombinant, full length GST fusion of Arabidopsis thaliana CBP80 Q9SIU2, At2g13540
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 200 µl
- Reconstitution: For reconstitution add 200 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: RNA Immunoprecipitation (RIP), Western blot (WB)
- Recommended dilutions: 5-10 µg (RIP), 1 : 1000 (WB)
- Expected | apparent MW: 96.5 kDa
- Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea, Pellia endiviifolia
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- CBP80 is a component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing. Alternative names: 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit, abscisic acid-hypersensitive protein 1, ABA-hypersensitive protein 1, Protein ENSALADA
- Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018. (RNA immunoprecipiation)Raczynska et al. (2013). TheSERRATEprotein isinvolved inalternativesplicing inArabidopsis thaliana. Nucleic Acids Res. Oct 16.
- Suggested blotting conditions: 8% gel, semi dry-blotting system, 25 V, 75 min, PVDF membrane
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