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{{/_source.additionalInfo}}Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase (goat antibody)
(Cat#: AS09 577)
Description
- Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
- Host: Goat
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 300 µl
- Reconstitution: For reconstitution add 300 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000-1 : 3000 (WB)
- Expected | apparent MW: 26 | 31 kDa (Arabidopsis thaliana)
- Confirmed reactivity: Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
- McLoughlin et al. (2012). TheSnf1-relatedproteinkinasesSnRK2.4 andSnRK2.10 areinvolved inmaintenance ofrootsystemarchitecture duringsaltstress. Plant J. June 2012.
- V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
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