Anti-HydA2 | Iron-hydrogenase HydA2

(Cat#: AS09 600)
AS09_600
AS09_600_1
Description
  • Immunogen: Recombinant, full-length Chlamydomonas reinhardtii HydA-2 UniProt: Q8VZZ0
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 100 µl
  • Reconstitution: For reconstitution add 100 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 5000 (WB)
  • Expected | apparent MW: 53.7 | 47.3 kDa (after a signal peptide is cleaved)
  • Confirmed reactivity: Chlamydomonas reinhardtii, recombinant HydA2 expressed in E.coli
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. Chlamydomonas the protein is present in low levels of 1 µg/liter of culture. Synonymnes: HYD2
  • HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa

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