Donkey anti-Sheep IgG (H&L), ALP conjugated

(Cat#: AS09 628)
AS09_628
AS09_628
Description
  • Immunogen: Purified sheep IgG, whole molecule
  • Host: Donkey
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified donkey IgG.
  • Format: Liquid
  • Quantity: 1 mg
  • Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
  • Tested applications: ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
  • Recommended dilutions: 1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA),1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
  • Confirmed reactivity: Sheep IgG heavy and light chains (H&L)
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
  • APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

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