Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to human, mouse,Rat IgG

(Cat#: AS10 1058)
AS10_1058
AS10_1058
Description
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified IgG, F(ab)'2 fragment.
  • Format: Liquid
  • Quantity: 0.5 mg
  • Storage: Store at 2-8°C. Shelf life of this product is one year from date of receipt.
  • Recommended dilutions: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC)
  • Rabbit anti-goat IgG (H&L) is a secondary antibody F(ab)'2 fragment, which binds to all goat IgGs in immunological assays and is FITC (fluorescein-5-isothiocyanate) conjugated. Antibody is also specially adsorbed against human,mouse and rat IgG. FITC Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE, may contain small amounts of intact IgG.
  • This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free, 0.05% (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

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