Donkey anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG

(Cat#: AS10 1115)
AS10_1115
AS10_1115
Description
  • Host: Donkey
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified IgG.
  • Format: Lyophilized
  • Quantity: 1 mg
  • Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
  • Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
  • Recommended dilutions: The optimal working dilution should be determined by the investigator
  • Donkey anti-rat IgG (H&L),HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays, except rat Ig1. Antibody is adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG, and affinity purified using solid phase rat IgG.
  • This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis, except rat Ig1.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

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