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{{/_source.additionalInfo}}Chicken anti-Mouse IgG (H&L), HRP conjugated, min, cross-reactivity to human, rabbit IgG/serum
(Cat#: AS10 1167)
Description
- Host: Chicken
- Clonality: Polyclonal
- Purity: Immunogen affinity purified IgY.
- Format: Lyophilized
- Quantity: 0.5 mg
- Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
- Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
- Recommended dilutions: The optimal working dilution should be determined by the investigator
- Chicken anti-mouse IgG (H&L),HRP conjugated, is a secondary antibody which binds to mouse IgGs in immunological asssyas. Antibodies are adsorbed against human,rabbit IgG/serum and affinity purified using solid phase mouse IgG.
- This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and human and rabbit IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10% (w/v) BSA, Protease/IgG free and 0.1% (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
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