Rabbit anti-Mouse IgG (H&L), ALP conjugated, min, cross-reactivity to human serum

(Cat#: AS10 1235)
AS10_1235
AS10_1235
Description
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified IgG.
  • Format: Liquid
  • Quantity: 1 mg
  • Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
  • Recommended dilutions: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
  • Rabbit anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase mouse IgG.
  • This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

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