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{{/_source.additionalInfo}}Goat anti-Rabbit IgG Fc, HRP conjugated
(Cat#: AS10 858)
Description
- Immunogen: Purified Rabbit IgG
- Host: Goat
- Clonality: Polyclonal
- Purity: Immunogen affinity purified goat IgG.
- Format: Lyophilized
- Quantity: 1 mg
- Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
- Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
- Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
- Recommended dilutions: The optimal working dilution should be determined by the investigator
- Confirmed reactivity: Rabbit IgG Fc (two heavy chains with constant domains)
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.
- HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
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