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{{/_source.additionalInfo}}Goat anti-Human IgG (H&L), F(ab)'2 fragment, TRITC conjugated
(Cat#: AS10 946)
Description
- Host: Goat
- Clonality: Polyclonal
- Purity: Immunogen affinity purified goat IgG, F(ab)'2 fragment.
- Format: Lyophilized
- Quantity: 0.5 mg
- Reconstitution: For reconstitution add 0,55 ml of sterile water
- Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
- Recommended dilutions: The optimal working dilution should be determined by the investigator,
- Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary, Rhodamine (TRITC-rhodamine - Tetramethylrhodamine-5-isothiocyanate) conjugated antibody, which is reacting against human IgG (H&L), F(ab)'2 fragment. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
- This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
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