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{{/_source.additionalInfo}}Anti-HSP18,5 | class IV heat shock protein
(Cat#: AS11 1628)
Description
- Immunogen: Recombinat Arabidopsis thaliana HSP18.5, expressed in E.coli, UniProt: O64564, TAIR: AT2G19310
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 200 µl
- Reconstitution: For reconstitution add 200 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 18.5 kDa
- Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare
- Not reactive in: Prosopis cineraria
- HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.
- Sadura et al. (2020). HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures . Int J Mol Sci . 2020 Mar 10;21(5):1889.doi: 10.3390/ijms21051889.
- Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01%. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
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