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{{/_source.additionalInfo}}Anti-GAI | DELLA protein GAI
(Cat#: AS11 1631)
Description
- Immunogen: KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence UniProt: Q9LQT8, TAIR: At1g14920
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 25 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB) on transfected Arabidopsis thaliana protoplasts
- Expected | apparent MW: 58.9 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: Brassica napus, Populus sp., Solanum lycopersicum
- GAI is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Less sensitive to GA. Synonyms: GRAS family protein 3, AtGRAS-3, Gibberelic acid-insensitive mutant protein, Restoration of growth on ammonia protein 2.
- Gorshkova & Pojidaeva (2021). Members of the Universal Stress Protein Family are Indirectly Involved in Gibberellin-Dependent Regulation of Germination and Post-Germination Growth. Russ J Plant Physiol 68, 451â??462 (2021). https://doi.org/10.1134/S1021443721030055Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016.
- Homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here. GAI protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.
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